Assume the following intracellular conditions: [ATP] = 15 mM, [ADP] = 0.5 mM, [Pi] = 7.9 mM, pH = 7.4, and temperature = 37°C. Given the ΔG°′ for ATP hydrolysis is −30.5 kJ/mol, what is the overall ΔG (kJ/mol) for ATP hydrolysis under these cellular conditions?
ANSWER
ΔG = −54.1 kJ/mol
| Gibbs free energy | standard conditions |
| ATP hydrolysis mechanism | temperature conversion |
| thermodynamic equations | thermodynamics |
| unit conversion |