Generate randomized peptide sequences with controlled length and ionizable side-chain composition. Toggle amino acids to customize the random pool, or build a sequence by hand. Output includes net charge at pH 7.0, isoelectric point, molecular weight, ε₂₈₀ for Beer-Lambert quantification, and a charge-vs-pH plot.

Amino Acid Pool
Standard
Ionizable (C D E H K R Y)
Disabled
Manual sequence builder

Switch to manual build, then type, paste, or click residues to assemble a sequence.

Randomizer Inputs
Number of amino acid residues (1-200)
Drawn from enabled ionizables: C D E H K R Y
Generate
Output
Sequence Analysis
No sequence yet. Set parameters and click Generate sequence.
Structure
Predominant at pH 7
Generate a sequence to see its skeletal structure with pH 7 protonation states.
Net charge via Henderson-Hasselbalch with terminal pKa from first/last residue · pKa values per Cochran reference · ε₂₈₀ via Edelhoch/Pace
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